hPU.1ΔN165-pET28b
(Plasmid #199796)

• Purpose: Expressed human PU.1/Spi-1 ETS domain (residues 165 to 270) in E. coli
• Depositing Lab: Gregory Poon
• Publication: Xhani et al Sci Adv. 2020 Feb 21;6(8):eaay3178. doi: 10.1126/sciadv.aay3178. eCollection 2020 Feb.

Backbone

• Vector backbone: pET28b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5246
• Total vector size (bp): 5368
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Induce with 0.5 mM IPTG at 30°C for protein expression.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: spi1
• Alt name: Spi-1 proto-oncogene
• Species: H. sapiens (human)
• Insert Size (bp): 326
• Entrez Gene: SPI1 (a.k.a. AGM10, OF, PU.1, SFPI1, SPI-1, SPI-A)
• Tag / Fusion Protein:
  • None (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7promoter
• 3′ sequencing primer: T7terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry