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pMSP1D1
(Plasmid #20061)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 20061 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MSP1D1
  • Alt name
    membrane scaffold protein
  • Alt name
    APOA1
  • Alt name
    PDB: 1AV1
  • Species
    synthetic gene
  • Insert Size (bp)
    640
  • Mutation
    deletion mutant (1-11) of MSP1; His-tag on N-terminus followed by spacer sequence and TEV protease site
  • Tag / Fusion Protein
    • 7-His tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nco I (not destroyed)
  • 3′ cloning site Hind III (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

NOTE: This plasmid is supplied in DH5alpha. For expression, it must be prepped and retransformed into BL-21(DE3); high oxygenation level

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSP1D1 was a gift from Stephen Sligar (Addgene plasmid # 20061 ; http://n2t.net/addgene:20061 ; RRID:Addgene_20061)
  • For your References section:

    Directed self-assembly of monodisperse phospholipid bilayer Nanodiscs with controlled size. Denisov IG, Grinkova YV, Lazarides AA, Sligar SG. J Am Chem Soc. 2004 Mar 24. 126(11):3477-87. 10.1021/ja0393574 PubMed 15025475