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pMSP1E3D1
(Plasmid #20066)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 20066 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET 28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL-21(DE3); high oxygenation level
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MSP1E3D1
  • Alt name
    membrane scaffold protein
  • Species
    synthetic gene
  • Insert Size (bp)
    840
  • Mutation
    "extended" MSP1D1; contains repeats of helices 4, 5 and 6;N-terminal 7-his tag followed by spacer sequence and TEV protease cleavage site
  • Tag / Fusion Protein
    • 7-His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nco I (not destroyed)
  • 3′ cloning site Hind III (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSP1E3D1 was a gift from Stephen Sligar (Addgene plasmid # 20066 ; http://n2t.net/addgene:20066 ; RRID:Addgene_20066)
  • For your References section:

    Cooperativity in cytochrome P450 3A4: linkages in substrate binding, spin state, uncoupling, and product formation. Denisov IG, Baas BJ, Grinkova YV, Sligar SG. J Biol Chem. 2007 Mar 9. 282(10):7066-76. 10.1074/jbc.M609589200 PubMed 17213193