Addgene - GFP-cdc42palm Plasmid Data

Plasmid 20141: GFP-cdc42palm

Gene/insert name:
cell division cycle 42 isoform 2
Alt name:
GFP-cdc42 WT palm
Insert size:
600
Species:
H. sapiens (human)
GenBank ID:
NP_426359
Entrez Gene:
CDC42 (RP1-224A6.5, CDC42Hs, G25K)
Fusion protein or tag:
GFP
Terminal:
N terminal on backbone
Mutation:
wild type
Vector backbone:
pEGFP-C1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
BglII
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
sequence List of Sequencing Primers
3' sequencing primer:
sequence
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
LB medium
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Alaa El-Husseini
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Neural palmitoyl-proteomics reveals dynamic synaptic palmitoylation. Kang et al (Nature. 2008 Dec 18. 456(7224):904-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20141" in your Materials and Methods section.