Addgene - YFP-tagged FRB (YR) Plasmid Data

Plasmid 20148: YFP-tagged FRB (YR)

Gene/insert name:
YFP-FRB (YR)
Species:
H. sapiens (human)
Vector backbone:
EYFP-C1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4733
Cloning site 5':
Xba1
Site destroyed during cloning:
Unknown
Cloning site 3':
Xba1
Site destroyed during cloning:
Unknown
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (3)
Principal Investigator:
Tobias Meyer
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: An inducible translocation strategy to rapidly activate and inhibit small GTPase signaling pathways. Inoue et al (Nat Methods. 2005 Jun . 2(6):415-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20148" in your Materials and Methods section.