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(Plasmid #20175)


Item Catalog # Description Quantity Price (USD)
Plasmid 20175 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4733
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    YFP-FKBP (YF):
  • Alt name
  • Species
    H. sapiens (human)
  • Mutation
    FKBP was taken out from pC4M-F2E with a flanking sequence encoding BsrGI and XhoI, along with a flexible linker sequence (agt gct ggt ggt agt gct ggt ggt agt gct ggt ggt agt gct ggt ggt agt gct ggt ggt) at the C terminus of FKBP, and then inserted into the YFP (C1) vector.
  • Entrez Gene
    FKBP1A (a.k.a. FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrG1 (unknown if destroyed)
  • 3′ cloning site Xho1 (unknown if destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YFP-FKBP (YF) was a gift from Tobias Meyer (Addgene plasmid # 20175 ; ; RRID:Addgene_20175)
  • For your References section:

    An inducible translocation strategy to rapidly activate and inhibit small GTPase signaling pathways. Inoue T, Heo WD, Grimley JS, Wandless TJ, Meyer T. Nat Methods. 2005 Jun . 2(6):415-8. 10.1038/nmeth763 PubMed 15908919