Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20170||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7000
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Kanamycin
Growth instructionsPlasmid is supplied already transformed into strain GT1265 (ruvAB recA) containing plasmid c1857 (which encodes a temperature-sensitive lambda repressor. Grow at 30°C. Supplement media with 100µg/ml ampicillin and 30µg/ml kanamycin to maintain both plasmids.
SpeciesA. thaliana (mustard weed); E. coli
Insert Size (bp)609
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGTI25 was a gift from Stephen West (Addgene plasmid # 20170 ; http://n2t.net/addgene:20170 ; RRID:Addgene_20170)
For your References section:Purification and properties of the RuvA and RuvB proteins of Escherichia coli. Tsaneva IR, Illing G, Lloyd RG, West SC. Mol Gen Genet. 1992 Oct . 235(1):1-10. 10.1007/BF00286175 PubMed 1435721