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pGTI25
(Plasmid #20170)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 20170 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCP3
  • Backbone manufacturer
    n/a
  • Backbone size w/o insert (bp) 7000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    GT1265
  • Growth instructions
    Plasmid is supplied already transformed into strain GT1265 (ruvAB recA) containing plasmid c1857 (which encodes a temperature-sensitive lambda repressor. Grow at 30°C. Supplement media with 100µg/ml ampicillin and 30µg/ml kanamycin to maintain both plasmids.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RuvA
  • Species
    A. thaliana (mustard weed); E. coli
  • Insert Size (bp)
    609
  • Mutation
    none
  • GenBank ID
    BAA01034

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Irina Tsaneva

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGTI25 was a gift from Stephen West (Addgene plasmid # 20170 ; http://n2t.net/addgene:20170 ; RRID:Addgene_20170)
  • For your References section:

    Purification and properties of the RuvA and RuvB proteins of Escherichia coli. Tsaneva IR, Illing G, Lloyd RG, West SC. Mol Gen Genet. 1992 Oct . 235(1):1-10. 10.1007/BF00286175 PubMed 1435721