pB-CAG-FLEX-F14F15-BFP-(mCherry)'
(Plasmid #201831)

• Purpose: piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase
• Depositing Lab: Ian Wickersham
• Publication: Jin et al Cell Rep Methods. 2023 Nov 20;3(11):100644. doi: 10.1016/j.crmeth.2023.100644.

Backbone

• Vector backbone: pB-CAG
• Backbone size w/o insert (bp): 6000
• Total vector size (bp): 8924
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: CAG
• Alt name: CAG promoter
• Species: Synthetic
• Insert Size (bp): 1699
• Promoter: CAG

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: BFP
• Alt name: mTagBFP2
• Species: Synthetic

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
  • piggyBac Limited Use Label License
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.02.23.481706v3 for bioRxiv preprint.