pUC19/PLIN2-GFP
(Plasmid #202194)

• Purpose: knock-in donor vector of GFP to the C-terminus of endogenous human PLIN2
• Depositing Lab: James Olzmann
• Publication: Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)00331-3. doi: 10.1016/j.devcel.2023.07.001.

Backbone

• Vector backbone: pUC19
• Backbone size w/o insert (bp): 2686
• Total vector size (bp): 4600
• Modifications to backbone: added C-terminal PLIN2 homology arms
• Vector type: cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PLIN2
• Alt name: GFP knock-in to C-terminus of PLIN2
• Species: H. sapiens (human)
• Insert Size (bp): 1897
• GenBank ID: NM_001122.4
• Entrez Gene: PLIN2 (a.k.a. ADFP, ADRP)
• Promoter: lac
• Tag / Fusion Protein:
  • GFP knock-in

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: M13/pUC Reverse
• 3′ sequencing primer: M13 (-21) Forward

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry