Addgene - pBABE HAII Plasmid Data

Plasmid 14738: pBABE HAII

Gene/insert name:
None
Fusion protein or tag:
HA
Terminal:
N terminal on backbone
Vector backbone:
pBABE puro
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size (bp):
5169
Cloning site 5':
See map
Site destroyed during cloning:
No
Cloning site 3':
See map
Site destroyed during cloning:
No
5' sequencing primer:
pBABE 5' List of Sequencing Primers
3' sequencing primer:
pBABE 3'
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Adrienne Cox
Terms and Licenses:
MTA

Comments:

The BamHI to SalI region of pBABE puro was replaced with an adaptor containing the HA tag and BamHI, EcoRI, and SalI sites.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Mammalian expression vectors for Ras family proteins: generation and use of expression constructs to analyze Ras family function. Fiordalisi et al (Methods Enzymol. 2001 . 332():3-36. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14738" in your Materials and Methods section.