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pTC-0X1L
(Plasmid #20315)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 20315 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Pmyc1tetO-lacZ
  • Species
    E. coli
  • Insert Size (bp)
    3842
  • Entrez Gene
    lacZ (a.k.a. ECIAI39_0334)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer NA
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTC-0X1L was a gift from Dirk Schnappinger (Addgene plasmid # 20315 ; http://n2t.net/addgene:20315 ; RRID:Addgene_20315)
  • For your References section:

    Improved tetracycline repressors for gene silencing in mycobacteria. Klotzsche M, Ehrt S, Schnappinger D. Nucleic Acids Res. 2009 Jan 27. ():. 10.1093/nar/gkp015 PubMed 19174563