Addgene - pTE-10M0X Plasmid Data

Plasmid 20318: pTE-10M0X

Gene/insert name:
Pimyc-tetR#10
Insert size:
970
Species:
synthetic gene derived from Tn10 tetR
Vector backbone:
pTE-mcs
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
5133
Cloning site 5':
PacI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Hygromycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Dirk Schnappinger
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Comments:

There are minor discrepancies between depositor's and Addgene's sequence. Depositor is aware of these and thinks they should not have any affect on function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Improved tetracycline repressors for gene silencing in mycobacteria. Klotzsche et al (Nucleic Acids Res. 2009 Jan 27. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20318" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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