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pWS-TK2
(Plasmid #20348)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 20348 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pWS-TK2
  • Species
    cloning vector
  • GenBank ID
    EU530623

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

The deposited sequence file of pWS-TK2 is from compilation of previous plasmids, and only part of it has been re-sequenced. There is a gap between author's sequence and Addgene sequence that will NOT affect the use of this plasmid.

Please also note that Addgene's quality control sequencing indicates that the PacI and SgfI sites present in the depositor's map are not present in the plasmid's actual sequence.

pWS-TK2 is designed to have two variants of thymidine kinase gene from herpes virus.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWS-TK2 was a gift from Mario Capecchi (Addgene plasmid # 20348 ; http://n2t.net/addgene:20348 ; RRID:Addgene_20348)
  • For your References section:

    A protocol for constructing gene targeting vectors: generating knockout mice for the cadherin family and beyond. Wu S, Ying G, Wu Q, Capecchi MR. Nat Protoc. 2008 . 3(6):1056-76. 10.1038/nprot.2008.70 PubMed 18546598