Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20379||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6470
Growth in Bacteria
Alt namesirtuin (silent mating type information regulation 2 homolog) 1
SpeciesH. sapiens (human)
Insert Size (bp)1420
Mutation3'UTR of SIRT1
Entrez GeneSIRT1 (a.k.a. SIR2, SIR2L1, SIR2alpha)
- Cloning method Restriction Enzyme
- 5′ cloning site PmeI (not destroyed)
- 3′ cloning site SacI (not destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
There are mismatches between Addgene's sequence and NCBI sequence for SIRT1 UTR. Deposit is aware of these changes and doesn't think they affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Luc-SIRT1 3'UTR was a gift from Charles Lowenstein (Addgene plasmid # 20379 ; http://n2t.net/addgene:20379 ; RRID:Addgene_20379)
For your References section:miR-34a repression of SIRT1 regulates apoptosis. Yamakuchi M, Ferlito M, Lowenstein CJ. Proc Natl Acad Sci U S A. 2008 Sep 9. 105(36):13421-6. 10.1073/pnas.0801613105 PubMed 18755897