Addgene - pTA-Luc SIRT1 promoter (-202) Plasmid Data

Plasmid 10971: pTA-Luc SIRT1 promoter (-202)

Gene/insert name:
SIRT1 promoter
Insert size:
202
Species:
M. musculus (mouse)
Entrez Gene:
Sirt1 (AA673258, SIR2L1, Sir2, Sir2a, Sir2alpha)
Vector backbone:
pTA-Luc
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
4800
5' sequencing primer:
RVprimer3 List of Sequencing Primers
3' sequencing primer:
LucNrev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Toren Finkel
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Nutrient availability regulates SIRT1 through a forkhead-dependent pathway. Nemoto et al (Science. 2004 Dec 17. 306(5704):2105-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10971" in your Materials and Methods section.