PE-Max-pet21A
(Plasmid #204471)

• Purpose: Expression plasmid for PE protein in bacteria.
• Depositing Lab: Scot Wolfe
• Publication: Ponnienselvan et al Nucleic Acids Res. 2023 May 29:gkad456. doi: 10.1093/nar/gkad456.

Backbone

• Vector backbone: pet21A
• Backbone manufacturer: NA
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 11907
• Vector type: Bacterial Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Prime editor
• Alt name: PEmax
• Species: bacteria
• Insert Size (bp): 6499
• Tag / Fusion Protein:
  • His-tag (C terminal on backbone)

Resource Information

• Supplemental Documents:
  • WOP-PE-V1.gbk
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry