Addgene - pWZL Neo Myr Flag NEK3 Plasmid Data

Plasmid 20547: pWZL Neo Myr Flag NEK3

Gene/insert name:
NEK3
Alt name:
NIMA (never in mitosis gene a)-related kinase 3
Species:
H. sapiens (human)
GenBank ID:
NM_002498
Entrez Gene:
NEK3 (HSPK36, MGC29949)
Fusion protein or tag:
Myr
Terminal:
N terminal on backbone
Fusion protein or tag:
Flag
Terminal:
N terminal on backbone
Vector backbone:
pWZL-Neo-Myr-Flag-DEST
(Search Vector Database)
Backbone manufacturer:
William Hahn Lab (available at Addgene, #15300)
Vector type:
Mammalian Expression, Retroviral
Cloning method:
Gateway Cloning
5' sequencing primer:
pWZL-Fwd (GAA CCT CCT CGT TCG ACC) List of Sequencing Primers
3' sequencing primer:
pWZL-Rev (TTC CGG GCC CTC ACA TTG)
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3 cells to prevent recombination.
High or low copy:
Unknown
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
4-C8
Sequence: View sequences (1)
Principal Investigator:
William Hahn
Principal Investigator:
Jean Zhao
Terms and Licenses:
MTA

Comments:

ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.

This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Integrative Genomic Approaches Identify IKBKE as a Breast Cancer Oncogene (Kinase Library Clones) Boehm et al (Cell. 2007 Jun 15. 129(6):1065-1079. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20547" in your Materials and Methods section.