pLVNP2.0-SpCas9
(Plasmid
#206880)
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PurposeExpresses FLAG-tagged SpCas9 fused to the N-terminus of Gag/GagPol harbouring an intervening phospholipase C-δ1 pleckstrin homology (PH) domain
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 206880 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLVNP2.0
- Total vector size (bp) 14588
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Vector typeLentiviral, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpCas9
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Insert Size (bp)4101
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Entrez Genecas9 (a.k.a. SD90_RS03825, SD90_03830)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer gcgctaccggTACCACCATG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVNP2.0-SpCas9 was a gift from Jacob Giehm Mikkelsen (Addgene plasmid # 206880 ; http://n2t.net/addgene:206880 ; RRID:Addgene_206880) -
For your References section:
Engineered lentivirus-derived nanoparticles (LVNPs) for delivery of CRISPR/Cas ribonucleoprotein complexes supporting base editing, prime editing and in vivo gene modification. Haldrup J, Andersen S, Labial ARL, Wolff JH, Frandsen FP, Skov TW, Rovsing AB, Nielsen I, Jakobsen TS, Askou AL, Thomsen MK, Corydon TJ, Thomsen EA, Mikkelsen JG. Nucleic Acids Res. 2023 Sep 8:gkad676. doi: 10.1093/nar/gkad676. 10.1093/nar/gkad676 PubMed 37678882
Map uploaded by the depositor.
