pAAV MP-84 mRFP
(Plasmid #206926)

• Purpose: The micro promoter MP-84 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (84 bp) allows delivery of large genes in AAV.
• Depositing Lab: Markus Grompe
• Publication: Chai et al Mol Ther Methods Clin Dev. 2023 May 17;29:504-512. doi: 10.1016/j.omtm.2023.05.013. eCollection 2023 Jun 8.

Backbone

• Vector backbone: pAAV MP-84
• Backbone manufacturer: Sunghee Chai, Markus Grompe
• Backbone size w/o insert (bp): 4231
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: mRFP
• Species: Synthetic
• Insert Size (bp): 678
• Promoter: MP-84

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (unknown if destroyed)
• 3′ cloning site: NsiI (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pAAV MP-84 mRFP

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Intron and WPRE are cloned to enhance expression of transgene and as a stuffer DNA. These elements are not required for the promoter activity.