pRRL MND WASp
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||36248||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepRRLSIN.cppt.PGK-GFP.WPRE Addgene 12252
Backbone manufacturerDidier Trono
- Backbone size w/o insert (bp) 6857
- Total vector size (bp) 8134
Modifications to backbonePGK GFP removed (EcoRV and BamHI)and replaced with a multiple cloning site for addition of promoter CDS cassettes
Growth in Bacteria
Copy numberLow Copy
Alt nameWiskott-Aldrich Syndrome Protein
SpeciesH. sapiens (human)
Insert Size (bp)1509
Entrez GeneWAS (a.k.a. IMD2, SCNX, THC, THC1, WASP, WASPA)
- Promoter MND
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer gatcacgagactagcctcgag
- 3′ sequencing primer caacgggccacaactcctc (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byBackbone is from Addgene plasmid 12252 (Didier Trono)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRRL MND WASp was a gift from David Rawlings (Addgene plasmid # 36248)
For your References section:Ubiquitous high-level gene expression in hematopoietic lineages provides effective lentiviral gene therapy of murine Wiskott-Aldrich Syndrome. Astrakhan A, Sather BD, Ryu BY, Khim S, Singh S, Humblet-Baron S, Ochs HD, Miao CH, Rawlings DJ. Blood. 2012 Mar 19. 10.1182/blood-2011-03-340711 PubMed 22431569
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.