Addgene - FUW-tetO-hSOX2 Plasmid Data

Plasmid 20724: FUW-tetO-hSOX2

Gene/insert name:
SOX2
Insert size:
966
Species:
H. sapiens (human)
Entrez Gene:
SOX2 (ANOP3, MCOPS3)
Vector backbone:
FUW
(Search Vector Database)
Backbone manufacturer:
Carlos Lois
Vector type:
Lentiviral
Backbone size w/o insert (bp):
8382
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
LNCX List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
TOP10, 37C
High or low copy:
High Copy
Selectable markers:
Zeocin
Sequence: View sequences (2)
Principal Investigator:
Rudolf Jaenisch
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A drug-inducible system for direct reprogramming of human somatic cells to pluripotency. Hockemeyer et al (Cell Stem Cell. 2008 Sep 11. 3(3):346-53. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20724" in your Materials and Methods section.