B-HiFi SpCas9
(Plasmid #207362)

• Purpose: Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
• Depositing Lab: Ervin Welker
• Publication: Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi: 10.1038/s41467-023-41393-5.

Backbone

• Vector backbone: pX330-like (without U6-sgRNA coding sequence)
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: B-HiFi SpCas9
• Species: S. pyogenes
• Insert Size (bp): 4251
• Mutation: R691A, amino acids 1005-1013 replaced with two glycine
• Promoter: CBh
• Tag / Fusion Protein:
  • 3xFLAG (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • B-HiFi SpCas9.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2022.08.16.504097 for bioRxiv preprint