A cleavage rule for selection of increased-fidelity SpCas9 variants with high efficiency and no detectable off-targets.
Kulcsar PI, Talas A, Ligeti Z, Toth E, Rakvacs Z, Bartos Z, Krausz SL, Welker A, Vegi VL, Huszar K, Welker E
Nat Commun. 2023 Sep 16;14(1):5746. doi: 10.1038/s41467-023-41393-5. PubMed Article
Kulcsar PI, Talas A, Ligeti Z, Toth E, Rakvacs Z, Bartos Z, Krausz SL, Welker A, Vegi VL, Huszar K, Welker E
Nat Commun. 2023 Sep 16;14(1):5746. doi: 10.1038/s41467-023-41393-5. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 126757 | HypaR-SpCas9 | Expression plasmid for human codon-optimized increased fidelity Hypa2SpCas9 (without U6-sgRNA coding sequence, with silent mutations) |
| 126764 | B-HypaR-SpCas9 | Expression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9. |
| 207361 | B-Sniper SpCas9 | Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant |
| 207362 | B-HiFi SpCas9 | Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant |
| 207363 | B-evoSpCas9-V495M | Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant |
| 207364 | B-evoSpCas9-N515Y | Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant |
| 207365 | B-evoSpCas9-E526K | Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant |
| 207366 | B-evoSpCas9-Q661R | Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant |
| 207367 | B-HeFSpCas9-A661R | Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant |
| 207368 | B-HeFSpCas9-A695Q | Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant |
| 207369 | B-HeFSpCas9-A848K | Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant |
| 207370 | B-HeFSpCas9-A926Q | Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant |
| 207371 | B-HeFSpCas9-A1003K | Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant |
| 207372 | B-HeFSpCas9-A1060R | Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant |
| 207373 | PET-WT SpCas9-NLS-6xHis | Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells |
| 207374 | PET-Sniper SpCas9-NLS-6xHis | Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells |
| 207375 | PET-Blackjack SpCas9-NLS-6xHis | Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells |
| 207376 | PET-HiFi SpCas9-NLS-6xHis | Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells |
| 207377 | PET-B-Sniper SpCas9-NLS-6xHis | Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells |
| 207378 | PET-B-HiFi SpCas9-NLS-6xHis | Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells |
| 207379 | PET-eSpCas9-NLS-6xHis | Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells |
| 207380 | PET-eSpCas9-plus-NLS-6xHis | Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells |
| 207381 | PET-SpCas9-HF1-plus-NLS-6xHis | Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells |
| 207382 | PET-SpCas9-HF1-NLS-6xHis | Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells |
| 207383 | PET-B-eSpCas9-NLS-6xHis | Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells |
| 207384 | PET-HypaSpCas9-NLS-6xHis | Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells |
| 207385 | PET-B-SpCas9-HF1-NLS-6xHis | Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells |
| 207386 | PET-B-HypaSpCas9-NLS-6xHis | Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells |
| 207387 | PET-HypaR-SpCas9-NLS-6xHis | Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells |
| 207388 | PET-B-HypaR-SpCas9-NLS-6xHis | Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells |
| 207389 | PET-evoSpCas9-NLS-6xHis | Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells |
| 207390 | PET-B-evoSpCas9-NLS-6xHis | Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells |
| 207391 | PET-HeFSpCas9-NLS-6xHis | Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells |
| 207392 | PET-B-HeFSpCas9-NLS-6xHis | Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells |