|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20737||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6256
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Alt nameepidermal growth factor receptor vIII
SpeciesH. sapiens (human)
MutationEGFR with a 267 amino acid deletion in the extracellular domain (constitutively active)
Entrez GeneEGFR (a.k.a. ERBB, ERBB1, HER1, NISBD2, PIG61, mENA)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer MSCV (Common Sequencing Primers)
Please note that Addgene's sequencing results identified potential polymorphisms L11F and C579Y in the EGFR sequence when compared to GenBank reference sequence NP_958439.1. The expressed protein functions as described in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MSCV-XZ066-EGFRvIII was a gift from Alonzo Ross (Addgene plasmid # 20737 ; http://n2t.net/addgene:20737 ; RRID:Addgene_20737)
For your References section:EGFRvIII expression and PTEN loss synergistically induce chromosomal instability and glial tumors. Li L, Dutra A, Pak E, Labrie JE, Gerstein RM, Pandolfi PP, Recht LD, Ross AH. Neuro Oncol. 2009 Feb . 11(1):9-21. 10.1215/15228517-2008-081 PubMed 18812521
Map uploaded by the depositor.