MSCV-XZ066-EGFRvIII
(Plasmid #20737)

• Depositing Lab: Alonzo Ross
• Publication: Li et al Neuro Oncol. 2009 Feb . 11(1):9-21.

Backbone

• Vector backbone: MSCV-XZ066
• Backbone size w/o insert (bp): 6256
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EGFRvIII
• Alt name: epidermal growth factor receptor vIII
• Species: H. sapiens (human)
• Mutation: EGFR with a 267 amino acid deletion in the extracellular domain (constitutively active)
• Entrez Gene: EGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: MSCV

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 14 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results identified potential polymorphisms L11F and C579Y in the EGFR sequence when compared to GenBank reference sequence NP_958439.1. The expressed protein functions as described in the associated publication.