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MSCV-XZ066-EGFRvIII
(Plasmid #20737)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 20737 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MSCV-XZ066
  • Backbone size w/o insert (bp) 6256
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EGFRvIII
  • Alt name
    epidermal growth factor receptor vIII
  • Species
    H. sapiens (human)
  • Mutation
    EGFR with a 267 amino acid deletion in the extracellular domain (constitutively active)
  • Entrez Gene
    EGFR (a.k.a. ERBB, ERBB1, HER1, NISBD2, PIG61, mENA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer MSCV
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please note that Addgene's sequencing results identified potential polymorphisms L11F and C579Y in the EGFR sequence when compared to GenBank reference sequence NP_958439.1. The expressed protein functions as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-XZ066-EGFRvIII was a gift from Alonzo Ross (Addgene plasmid # 20737 ; http://n2t.net/addgene:20737 ; RRID:Addgene_20737)
  • For your References section:

    EGFRvIII expression and PTEN loss synergistically induce chromosomal instability and glial tumors. Li L, Dutra A, Pak E, Labrie JE, Gerstein RM, Pandolfi PP, Recht LD, Ross AH. Neuro Oncol. 2009 Feb . 11(1):9-21. 10.1215/15228517-2008-081 PubMed 18812521