pIG-829_HA-GD2-28z_CAR_JUN_Retroviral
(Plasmid
#207506)
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PurposeThis plasmid can be used to generate retrovirus.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 207506 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonePMSGV
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Vector typeRetroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameJUN, HA-GD2-28z_CAR
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SpeciesH. sapiens (human)
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Entrez GeneJUN (a.k.a. AP-1, AP1, c-Jun, cJUN, p39)
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byHA-GD2-28z CAR sequence and MSGV backbone were provided by Crystal Mackall/Robbie Majzner.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2022.07.27.501186 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIG-829_HA-GD2-28z_CAR_JUN_Retroviral was a gift from Alexander Marson (Addgene plasmid # 207506 ; http://n2t.net/addgene:207506 ; RRID:Addgene_207506) -
For your References section:
Modular pooled discovery of synthetic knockin sequences to program durable cell therapies. Blaeschke F, Chen YY, Apathy R, Daniel B, Chen AY, Chen PA, Sandor K, Zhang W, Li Z, Mowery CT, Yamamoto TN, Nyberg WA, To A, Yu R, Bueno R, Kim MC, Schmidt R, Goodman DB, Feuchtinger T, Eyquem J, Jimmie Ye C, Carnevale J, Satpathy AT, Shifrut E, Roth TL, Marson A. Cell. 2023 Sep 14;186(19):4216-4234.e33. doi: 10.1016/j.cell.2023.08.013. 10.1016/j.cell.2023.08.013 PubMed 37714135
