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Modular pooled discovery of synthetic knockin sequences to program durable cell therapies.
Blaeschke F, Chen YY, Apathy R, Daniel B, Chen AY, Chen PA, Sandor K, Zhang W, Li Z, Mowery CT, Yamamoto TN, Nyberg WA, To A, Yu R, Bueno R, Kim MC, Schmidt R, Goodman DB, Feuchtinger T, Eyquem J, Jimmie Ye C, Carnevale J, Satpathy AT, Shifrut E, Roth TL, Marson A
Cell. 2023 Sep 14;186(19):4216-4234.e33. doi: 10.1016/j.cell.2023.08.013. PubMed Article

Plasmids from Article

ID Plasmid Purpose
207474plIG-106_NY-ESO-1_TCR_SR_LibraryNY-ESO-1 TCR in combination with 129 surface receptors and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the TRAC locus of human T cells
207475plIG-107_NY-ESO-1_TCR_TF_LibraryNY-ESO-1 TCR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the
207476plIG-109_CD19-BBz_CAR_SR_LibraryCD19-BBz CAR in combination with 129 surface receptors and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the TRAC locus of human T cells
207477plIG-110_CD19-BBz_CAR_TF_LibraryCD19-BBz CAR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the
207478plIG-123_HA-GD2-28z_CAR_TF_LibraryHA-GD2-28z CAR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into th
207479plIG-124_HA-GD2-28z_CAR_SR_LibraryHA-GD2-28z CAR in combination with 129 surface receptors and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the TRAC locus of human T cel
207480plIG-128_HA-GD2-28z_CAR_TFxTF_LibraryHA-GD2-28z CAR in combination with ~10,000 transcription factor combinations (and related proteins) and controls (GFP/RFP combinations). Library can be used to generate HDR templates for modular poole
207481plIG-132_CD19-28z_CAR_TF_and_SR_LibraryCD19-28z CAR in combination with 129 surface receptors, 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knoc
207482pIG-601_NY-ESO-1_TCR_GFPThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
207483pIG-602_NY-ESO-1_TCR_RFPThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
207484pIG-671_HA-GD2-28z_CAR_tNGFRThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
207485pIG-702_CD19-BBz_CAR_tNGFRThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
207486pIG-704_CD19-28z_CAR_tNGFRThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
207487pIG-727_HA-GD2-28z_CAR_RFP-tNGFRThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
207488pIG-748_HA-GD2-28z_CAR_BATFThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
207489pIG-747_CD19-28z_CAR_TFAP4This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
207490pIG-749_HA-GD2-28z_CAR_TFAP4This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
207491pIG-804_HA-GD2-28z_CAR_BATF-TFAP4This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
207492pIG-805_HA-GD2-28z_CAR_BATF-RFPThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
207493pIG-806_HA-GD2-28z_CAR_RFP-TFAP4This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
207494pIG-755_HA-GD2-28z_CAR_RFP-JUNThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
207495pIG-832_NY-ESO-1_TCR_FAS/4-1BBThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
207496pIG-833_NY-ESO-1_TCR_FAS/CD28This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
207497pIG-834_NY-ESO-1_TCR_FAS/IL4RThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
207498pIG-835_NY-ESO-1_TCR_FAS/MyD88This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
207499pIG-836_NY-ESO-1_TCR_FAS/OX40This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
207500pIG-837_NY-ESO-1_TCR_tFASThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
207501pIG-851_HA-GD2-28z_HA-TFAP4This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
207502pIG-852_HA-GD2-28z_TFAP4-HAThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
207503pIG-858_CD19-BBz_CAR_TFAP4This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
207504pIG-874_HA-GD2-28z_CAR_IL2RAThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
207505pIG-828_HA-GD2-28z_CAR_BATF_RetroviralThis plasmid can be used to generate retrovirus.
207506pIG-829_HA-GD2-28z_CAR_JUN_RetroviralThis plasmid can be used to generate retrovirus.
207507pIG-830_HA-GD2-28z_CAR_tNGFR_RetroviralThis plasmid can be used to generate retrovirus.
207508pIG-831_HA-GD2-28z_CAR_TFAP4_RetroviralThis plasmid can be used to generate retrovirus.

Antibodies from Article