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BAT-lux
(Plasmid #20890)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 20890 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3basic
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    7xTCF binding sites, Siamois minimal promoter
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    350
  • Mutation
    7 multimerized TCF-binding elements fused upstream of the xSiamois minimal promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Wnt/beta-catenin responsive promoter in Xenopus embryos and human cell lines

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BAT-lux was a gift from Stefano Piccolo (Addgene plasmid # 20890 ; http://n2t.net/addgene:20890 ; RRID:Addgene_20890)
  • For your References section:

    Mapping Wnt/beta-catenin signaling during mouse development and in colorectal tumors. Maretto S, Cordenonsi M, Dupont S, Braghetta P, Broccoli V, Hassan AB, Volpin D, Bressan GM, Piccolo S. Proc Natl Acad Sci U S A. 2003 Mar 18. 100(6):3299-304. 10.1073/pnas.0434590100 PubMed 12626757