Addgene - pEP4 E02S EM2K Plasmid Data

Plasmid 20923: pEP4 E02S EM2K

Gene/insert name:
Oct4 and Sox2; Myc and Klf4
Insert size:
2700
Species:
H. sapiens (human)
GenBank ID:
NM_002701 NM_004235 NM_002467 NM_003106
Entrez Gene:
SOX2 (ANOP3, MCOPS3)
KLF4 (RP11-150J11.1, EZF, GKLF)
MYC (MRTL, bHLHe39, c-Myc)
Vector backbone:
pCEP4
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Episomal/EBNA
Backbone size w/o insert (bp):
12600
Cloning site 5':
multiple
Site destroyed during cloning:
Unknown
Cloning site 3':
multiple
Site destroyed during cloning:
Unknown
5' sequencing primer:
na List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Top10F'
Growth temperature (℃):
37
Growth instructions:
Top10 "Extremely" low copy number
High or low copy:
Low Copy
Sequence: View sequences (8)
Map:
View map
Principal Investigator:
James Thomson
Terms and Licenses:
MTA

Comments:

Oct4 and Sox2 are coexpressed under a strong EF promoter. Myc and Klf4 are coexpressed under a separate (but identical) EF promoter. This plasmid contains EBNA-1 and OriP for limited maintenance in mammalian cells.

These plasmids were used at concentrations of >1.5 mg/ml. When preparing these plasmids for transfection, 3 low concentration maxipreps are done and the elution is combined in about 700 ul.

Note that this a K278M mutation in Klf4, but it does not affect the reprogramming ability.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences Yu et al (Science. 2009 Mar 26. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20923" in your Materials and Methods section.