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pCEP4-M2L
(Plasmid #20926)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 20926 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCEP4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 10200
  • Vector type
    Episomal/EBNA
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    "Extremely" low copy number
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    MYC
  • Alt name
    bHLEHE39
  • Alt name
    c-Myc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • GenBank ID
    NM_002467.4
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
  • Promoter CMV

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer IRES-R2 (gacggcaatatggtggaaa)
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    LIN28
  • Alt name
    CSDD1
  • Alt name
    FLJ12457
  • Alt name
    LIN-28
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    600
  • GenBank ID
    NM_024674.4
  • Entrez Gene
    LIN28A (a.k.a. CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)
  • Promoter IRES

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer IRES-F
  • 3′ sequencing primer EBV_rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Myc and Lin28 are coexpressed under a strong CMV promoter. This plasmid contains EBNA-1 and OriP for limited maintenance in mammalian cells.

These plasmids were used at concentrations of >1.5 mg/ml. When preparing these plasmids for transfection, 3 low concentration maxipreps are done and the elution is combined in about 700 ul.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEP4-M2L was a gift from James Thomson (Addgene plasmid # 20926)
  • For your References section:

    Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences. Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA. Science. 2009 Mar 26. 10.1126/science.1172482 PubMed 19325077