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Purpose3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activation
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 20945 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLenti-SynapsinI
- Backbone size w/o insert (bp) 9000
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namechannelrhodopsin-2
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Alt nameChR2
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Alt namecop4
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Alt namechannelopsin-2
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SpeciesC. reinhardtii
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Insert Size (bp)1662
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MutationH134R mutation in ChR2
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
Depositor Comments
Please see http://www.optogenetics.org
for additional information.
The H134R mutation present in the ChR2 sequence is a gain-of-function mutant that produces larger stationary photocurrents in comparison to the wild-type ChR2 sequence
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE was a gift from Karl Deisseroth (Addgene plasmid # 20945 ; http://n2t.net/addgene:20945 ; RRID:Addgene_20945) -
For your References section:
Multimodal fast optical interrogation of neural circuitry. Zhang F, Wang LP, Brauner M, Liewald JF, Kay K, Watzke N, Wood PG, Bamberg E, Nagel G, Gottschalk A, Deisseroth K. Nature. 2007 Apr 5. 446(7136):633-9. 10.1038/nature05744 PubMed 17410168