Addgene - pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE Plasmid Data

Plasmid 20945: pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE

Purpose:
3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activation
Gene/insert name:
channelrhodopsin-2
Alt name:
ChR2
Alt name:
cop4
Alt name:
channelopsin-2
Insert size:
1662
Species:
C. reinhardtii
Mutation:
H134R mutation in ChR2
Vector backbone:
pLenti-SynapsinI
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
9000
Cloning site 5':
AgeI
Site destroyed during cloning:
Unknown
Cloning site 3':
EcoRI
Site destroyed during cloning:
Unknown
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Supplemental document:
Notes from Addgene (2)
Principal Investigator:
Karl Deisseroth
Terms and Licenses:
UBMTA
Ancillary Agreement for Plasmids Containing FP Materials

Comments:

for additional information.

The H134R mutation present in the ChR2 sequence is a gain-of-function mutant that produces larger stationary photocurrents in comparison to the wild-type ChR2 sequence

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Multimodal fast optical interrogation of neural circuitry. Zhang et al (Nature. 2007 Apr 5. 446(7136):633-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20945" in your Materials and Methods section.