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Plasmid 20945: pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE
  • 3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activation

  • channelrhodopsin-2

  • ChR2

  • cop4

  • channelopsin-2

  • 1662

  • C. reinhardtii

  • H134R mutation in ChR2

  • pLenti-SynapsinI
    (Search Vector Database)

  • Mammalian Expression, Lentiviral

  • 9000

  • AgeI

  • Unknown

  • EcoRI

  • Unknown

  • n/a List of Sequencing Primers

  • Ampicillin

  • Stbl3

  • 37

  • High Copy

  • View sequences (2)
  • Notes from Addgene (2)

  • Karl Deisseroth

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

Please see http://www.optogenetics.org

for additional information.

The H134R mutation present in the ChR2 sequence is a gain-of-function mutant that produces larger stationary photocurrents in comparison to the wild-type ChR2 sequence

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Multimodal fast optical interrogation of neural circuitry. Zhang et al (Nature. 2007 Apr 5. 446(7136):633-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20945" in your Materials and Methods section.