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PurposeMammalian expression of VChR1 fused to EYFP
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 20955 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5545
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namevolvox channelrhodopsin-1
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Alt nameVChR1
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SpeciesV. carteri
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Insert Size (bp)1626
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Tag
/ Fusion Protein
- EYFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1/VChR1-EYFP was a gift from Karl Deisseroth (Addgene plasmid # 20955 ; http://n2t.net/addgene:20955 ; RRID:Addgene_20955) -
For your References section:
Red-shifted optogenetic excitation: a tool for fast neural control derived from Volvox carteri. Zhang F, Prigge M, Beyriere F, Tsunoda SP, Mattis J, Yizhar O, Hegemann P, Deisseroth K. Nat Neurosci. 2008 Jun . 11(6):631-3. 10.1038/nn.2120 PubMed 18432196