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pmStrawberry-Atg4BC74A
(Plasmid #21076)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21076 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pmStrawberry C1
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Atg4B(C74A)
  • Alt name
    Atg4B
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1194
  • Mutation
    220-222 TGC(Cys) is changed to GCA (Ala)
  • GenBank ID
    NM_174874
  • Entrez Gene
    Atg4b (a.k.a. 2510009N07Rik, AW048066, Apg, Apg4b, Atg4bl, Autl1)
  • Tag / Fusion Protein
    • mStrawberry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY. Nat Biotechnol. 2004 Dec;22(12):1567-72. Epub 2004 Nov 21.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein.

Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY. Nat Biotechnol. 2004 Dec;22(12):1567-72.

Atg4B insert also contains V190A and T325K compared to listed reference sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmStrawberry-Atg4BC74A was a gift from Tamotsu Yoshimori (Addgene plasmid # 21076 ; http://n2t.net/addgene:21076 ; RRID:Addgene_21076)
  • For your References section:

    An Atg4B mutant hampers the lipidation of LC3 paralogues and causes defects in autophagosome closure. Fujita N, Hayashi-Nishino M, Fukumoto H, Omori H, Yamamoto A, Noda T, Yoshimori T. Mol Biol Cell. 2008 Nov . 19(11):4651-9. 10.1091/mbc.e08-03-0312 PubMed 18768752