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(Plasmid #21170)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 21170 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5256
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    E2F transcription factor 1
  • Alt name
    E2F 3'UTR WT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    E2F1 (a.k.a. E2F-1, RBAP1, RBBP3, RBP3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer GL2sequencing2
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-Control-E2F-3'UTR-WT was a gift from Joshua Mendell (Addgene plasmid # 21170)
  • For your References section:

    c-Myc-regulated microRNAs modulate E2F1 expression. O'Donnell KA, Wentzel EA, Zeller KI, Dang CV, Mendell JT. Nature. 2005 Jun 9. 435(7043):839-43. 10.1038/nature03677 PubMed 15944709