PurposeMammalian expression of human E2F1 with HA tag
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24225||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6200
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1300
Entrez GeneE2F1 (a.k.a. E2F-1, RBAP1, RBBP3, RBP3)
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer Bglob-intron-F
- 3′ sequencing primer Bglob-pA-R (Common Sequencing Primers)
Insert check with SalI, which leads to a 1.3kb fragment + vector. E2F1 cDNA can be removed by BamHI digest.
This plasmid contains an E356K mutation within the E2F1 insert. Please note that other mutations may exist and it is unclear whether this mutation affects plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMVHA E2F1 was a gift from Kristian Helin (Addgene plasmid # 24225 ; http://n2t.net/addgene:24225 ; RRID:Addgene_24225)
For your References section:Deregulated expression of E2F family members induces S-phase entry and overcomes p16INK4A-mediated growth suppression. Lukas J, Petersen BO, Holm K, Bartek J, Helin K. Mol Cell Biol. 1996 Mar . 16(3):1047-57. PubMed 8622649