Addgene - pCMVHA E2F1 Plasmid Data

Plasmid 24225: pCMVHA E2F1

Gene/insert name:
E2F1
Alt name:
E2F-1
Insert size:
1300
Species:
H. sapiens (human)
Entrez Gene:
E2F1 (E2F-1, RBAP1, RBBP3, RBP3)
Fusion protein or tag:
HA
Terminal:
N terminal on insert
Vector backbone:
pCMVHANeoBam
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6200
Cloning site 5':
BamHI
Site destroyed during cloning:
Unknown
Cloning site 3':
BamHI
Site destroyed during cloning:
Unknown
5' sequencing primer:
Bglob-intron-F List of Sequencing Primers
3' sequencing primer:
Bglob-pA-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
Kristian Helin
Terms and Licenses:
MTA

Comments:

Insert check with SalI, which leads to a 1.3kb fragment + vector. E2F1 cDNA can be removed by BamHI digest.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Deregulated expression of E2F family members induces S-phase entry and overcomes p16INK4A-mediated growth suppression. Lukas et al (Mol Cell Biol. 1996 Mar . 16(3):1047-57. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24225" in your Materials and Methods section.