Addgene - pCMVHA E2F2 Plasmid Data

Plasmid 24226: pCMVHA E2F2

Gene/insert name:
E2F2
Alt name:
E2F-2
Insert size:
1300
Species:
H. sapiens (human)
Entrez Gene:
E2F2 (E2F-2)
Fusion protein or tag:
HA
Terminal:
N terminal on insert
Vector backbone:
pCMVHANeoBam
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6600
Cloning site 5':
BamHI
Site destroyed during cloning:
Unknown
Cloning site 3':
BamHI
Site destroyed during cloning:
Unknown
5' sequencing primer:
Bglob-intron-F List of Sequencing Primers
3' sequencing primer:
Bglob-pA-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Principal Investigator:
Kristian Helin
Terms and Licenses:
MTA

Comments:

Insert check with SalI, which leads to three fragments 4.7kb 1,8kb 0,35kb. E2F1 cDNA can be removed by BamHI digest.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Deregulated expression of E2F family members induces S-phase entry and overcomes p16INK4A-mediated growth suppression. Lukas et al (Mol Cell Biol. 1996 Mar . 16(3):1047-57. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24226" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

24225: pCMVHA E2F1

24213: pCMVHA-E2F5

10914: pcDNA3-E2F4

37970: pCMV-Neo-Bam E2F3a

16007: pMax-E2F1