Addgene - pCMV-Neo-Bam E2F3a Plasmid Data

Plasmid 37970: pCMV-Neo-Bam E2F3a

Gene/insert name:
E2F3a
Insert size:
1450
Species:
H. sapiens (human)
Entrez Gene:
E2F3 (RP1-177P22.2, E2F-3)
Vector backbone:
pCMV-Neo-Bam
(Search Vector Database)
Backbone manufacturer:
Vogelstein Lab
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6600
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
Bglob-intron-F List of Sequencing Primers
3' sequencing primer:
Bglob-pA-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
Jacqueline Lees
Terms and Licenses:
MTA

Comments:

E2Fs bind DNA as a heterodimer with members of the DP family of transcription factors.

The 5' end of the coding sequence can be difficult to sequence.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The retinoblastoma protein binds to a family of E2F transcription factors. Lees et al (Mol Cell Biol. 1993 Dec;13(12):7813-25. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 37970" in your Materials and Methods section.