Addgene - GFP-pcDNA3-PKCgamma-cys1Acys1B Plasmid Data

Plasmid 21190: GFP-pcDNA3-PKCgamma-cys1Acys1B

Gene/insert name:
PKCgamma-cys1Acys1B
Insert size:
1089
Species:
R. norvegicus (rat)
Entrez Gene:
Prkcg (PKC, PKCI, Prkc, Prkcc, RATPKCI)
Fusion protein or tag:
GFP
Mutation:
PKCgamma-cys1Acys1B (aa35 to aa152 of rat PKCgamma). (Lab plasmid WC 0015)
Vector backbone:
pcDNA3
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5439
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
Tobias Meyer
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Green fluorescent protein (GFP)-tagged cysteine-rich domains from protein kinase C as fluorescent indicators for diacylglycerol signaling in living cells. Oancea et al (J Cell Biol. 1998 Feb 9. 140(3):485-98. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21190" in your Materials and Methods section.