Krox20 promoter pGL3-TATA
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21260||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4818
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameKrox20 promoter
SpeciesM. musculus (mouse)
Insert Size (bp)1341
Entrez GeneEgr2 (a.k.a. Egr-, Egr-2, Krox, Krox-, Krox-20, Krox20, NGF1-, NGF1-B, Zfp-2, Zfp-25, Zfp-6)
- Cloning method Restriction Enzyme
- 5′ cloning site Kpn1 (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
There is a T to C mismatch with Krox20. The single mis-match of the nucleotide is likely to reflect the polymorphism of different mouse strain used in the cloning.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Krox20 promoter pGL3-TATA was a gift from Jerry Crabtree (Addgene plasmid # 21260 ; http://n2t.net/addgene:21260 ; RRID:Addgene_21260)
For your References section:Calcineurin/NFAT signaling is required for neuregulin-regulated Schwann cell differentiation. Kao SC, Wu H, Xie J, Chang CP, Ranish JA, Graef IA, Crabtree GR. Science. 2009 Jan 30. 323(5914):651-4. 10.1126/science.1166562 PubMed 19179536