|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21261||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4818
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameKrox20-Myelin Specific Enhancer
SpeciesM. musculus (mouse)
Insert Size (bp)1293
Entrez GeneEgr2 (a.k.a. Egr-2, Krox-20, Krox20, NGF1-B, Zfp-25, Zfp-6)
- Cloning method Restriction Enzyme
- 5′ cloning site Kpn1 (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
Plasmid 21261: Krox20-MSE8 pGL3-TATA sequence match with genomic sequences on Chromosome 10 upstream of Egr2. It's an enhancer of Egr2 localized ~35 kb downstream of the defined genomic sequence of Egr2. If comparing the genomic location of plasmid 21261 (Chromosome 10: 9367948-9368511) and plasmid 21260 (Chromosome 10: 9331837-9332418), one can see that it's approximately 35 kb apart.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Krox20-MSE8 pGL3-TATA was a gift from Jerry Crabtree (Addgene plasmid # 21261 ; http://n2t.net/addgene:21261 ; RRID:Addgene_21261)
For your References section:Calcineurin/NFAT signaling is required for neuregulin-regulated Schwann cell differentiation. Kao SC, Wu H, Xie J, Chang CP, Ranish JA, Graef IA, Crabtree GR. Science. 2009 Jan 30. 323(5914):651-4. 10.1126/science.1166562 PubMed 19179536
Map uploaded by the depositor.