3HA-ERHBD-Hoxb8
(Plasmid #212932)

• Purpose: Expresses an estrogen-regulated form of Hoxb8 to generate conditionally immortalized hematopoietic progenitor cell lines
• Depositing Lab: Hans Haecker
• Publication: Redecke et al Nat Methods. 2013 Aug;10(8):795-803. doi: 10.1038/nmeth.2510. Epub 2013 Jun 9.

Backbone

• Vector backbone: MSCV
• Total vector size (bp): 8301
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3HA-ERHBD-Hoxb8
• Species: H. sapiens (human), M. musculus (mouse)
• Insert Size (bp): 1788
• Entrez Gene: ESR1 (a.k.a. ER, ESR, ESRA, ESTRR, Era, NR3A1)
• Entrez Gene: Hoxb8 (a.k.a. Hox-2.4)
• Promoter: MSCV LTR
• Tag / Fusion Protein:
  • Triple HA tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: MSCV-F
• 3′ sequencing primer: MSCV-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry