Addgene - pLKO.1 shMTH1-2 Plasmid Data

Plasmid 21298: pLKO.1 shMTH1-2

Gene/insert name:
shMTH1-2
Alt name:
MTH1 shRNA
Alt name:
MTH1
Species:
H. sapiens (human)
Entrez Gene:
NUDT1 (MTH1)
Vector backbone:
pLKO.1 puro
(Search Vector Database)
Backbone manufacturer:
Available at Addgene (#8453)
Vector type:
Mammalian Expression, Lentiviral, RNAi
Backbone size w/o insert (bp):
7000
Cloning site 5':
AgeI
Site destroyed during cloning:
Unknown
Cloning site 3':
EcoRI
Site destroyed during cloning:
Unknown
5' sequencing primer:
LKO.1 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Supplemental document:
Addgene's pLKO.1 protocol
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

shRNA #2 directed against MTH1 sequence 5-GAAATTCCACGGGTACTTCAA-3′.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Continuous elimination of oxidized nucleotides is necessary to prevent rapid onset of cellular senescence. Rai et al (Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):169-74. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21298" in your Materials and Methods section.