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pLew100::NLS-ISceI-HA
(Plasmid #21299)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21299 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLew100
  • Backbone size w/o insert (bp) 4300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    I-SceI
  • Insert Size (bp)
    2000
  • GenBank ID
    FJ797515
  • Entrez Gene
    SCEI (a.k.a. Q0160)
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

There are a few discrepancies between Addgene's sequence and the provided sequence from the depositing lab. These discrepancies do not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLew100::NLS-ISceI-HA was a gift from Nina Papavasiliou (Addgene plasmid # 21299)
  • For your References section:

    A yeast-endonuclease-generated DNA break induces antigenic switching in Trypanosoma brucei. Boothroyd CE, Dreesen O, Leonova T, Ly KI, Figueiredo LM, Cross GA, Papavasiliou FN. Nature. 2009 Apr 15. ():. 10.1038/nature07982 PubMed 19369939