Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21306||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4818
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameVEGFR2 promoter
SpeciesH. sapiens (human)
Insert Size (bp)493
Entrez GeneKDR (a.k.a. CD309, FLK1, VEGFR, VEGFR2)
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
-225 to +268 region of the promoter.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL3-VEGFR2-225 was a gift from Donald Ingber (Addgene plasmid # 21306 ; http://n2t.net/addgene:21306 ; RRID:Addgene_21306)
For your References section:A mechanosensitive transcriptional mechanism that controls angiogenesis. Mammoto A, Connor KM, Mammoto T, Yung CW, Huh D, Aderman CM, Mostoslavsky G, Smith LE, Ingber DE. Nature. 2009 Feb 26. 457(7233):1103-8. 10.1038/nature07765 PubMed 19242469