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pHR'-NRSF-CITE-GFP
(Plasmid #21310)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21310 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHR'
  • Backbone manufacturer
    Didier Trono
  • Backbone size w/o insert (bp) 10000
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    neuron restrictive silencer factor
  • Alt name
    NRSF, REST
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3664
  • Mutation
    None
  • GenBank ID
    NM_011263
  • Entrez Gene
    Rest (a.k.a. 2610008J04Rik, AA407358, D14Mgi1, N, NRSF, REST4)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (bicistronic with CITE/IRES)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer IRES-R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gift of David Anderson, Caltech reference SHIMOJO M, PAQUETTE AJ, ANDERSON DJ, AND HERSH LB. Protein kinase A regulates cholinergic gene expression in PC12 cells: REST4 silences the silencing activity of neuron-restrictive silencer factor/REST. Mol Cell Biol 19: 6788 – 6795, 1999.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR'-NRSF-CITE-GFP was a gift from Jay Nadeau (Addgene plasmid # 21310 ; http://n2t.net/addgene:21310 ; RRID:Addgene_21310)
  • For your References section:

    NRSF causes cAMP-sensitive suppression of sodium current in cultured hippocampal neurons. Nadeau H, Lester HA. J Neurophysiol. 2002 Jul . 88(1):409-21. 10.1152/jn.2002.88.1.409 PubMed 12091564