Addgene - pHR'-NRSF-CITE-GFP Plasmid Data

Plasmid 21310: pHR'-NRSF-CITE-GFP

Gene/insert name:
neuron restrictive silencer factor
Alt name:
NRSF, REST
Insert size:
3664
Species:
M. musculus (mouse)
GenBank ID:
NM_011263
Entrez Gene:
Rest (2610008J04Rik, AA407358, NRSF)
Fusion protein or tag:
GFP (bicistronic with CITE/IRES)
Mutation:
None
Vector backbone:
pHR'
(Search Vector Database)
Backbone manufacturer:
Didier Trono
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
10000
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Top10
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
Gift of David Anderson, Caltech reference SHIMOJO M, PAQUETTE AJ, ANDERSON DJ, AND HERSH LB. Protein kinase A regulates cholinergic gene expression in PC12 cells: REST4 silences the silencing activity of neuron-restrictive silencer factor/REST. Mol Cell Biol 19: 6788 – 6795, 1999.
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Jay Nadeau
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: NRSF causes cAMP-sensitive suppression of sodium current in cultured hippocampal neurons. Nadeau et al (J Neurophysiol. 2002 Jul . 88(1):409-21. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21310" in your Materials and Methods section.