|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21315||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepMXs MoMLV-based retroviral vector
- Backbone size w/o insert (bp) 4582
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namemonomeric Red Fluorescence Protein 1
Insert Size (bp)754
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HincII/EcoRV (destroyed during cloning)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
mRFP1 (maximum Excitation wave length, 584 nm; maximum Emission wave length, 607 nm). pMXs MoMLV-based retroviral vector from Dr. Toshio Kitamura (Institute of Medical Science, University of Tokyo).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMXs-mRFP1 was a gift from James Ellis (Addgene plasmid # 21315)
For your References section:Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency. Hotta A, Cheung AY, Farra N, Vijayaragavan K, Seguin CA, Draper JS, Pasceri P, Maksakova IA, Mager DL, Rossant J, Bhatia M, Ellis J. Nat Methods. 2009 May . 6(5):370-6. 10.1038/nmeth.1325 PubMed 19404254