Addgene - pHR'-ROMK1-CITE-GFP Plasmid Data

Plasmid 21322: pHR'-ROMK1-CITE-GFP

Gene/insert name:
ROMK1
Insert size:
3375
Species:
R. norvegicus (rat)
GenBank ID:
NM_017023
Entrez Gene:
Kcnj1 (Kcnj, ROMK1)
Fusion protein or tag:
GFP (bicistronic with CITE/IRES)
Vector backbone:
pHR'
(Search Vector Database)
Backbone manufacturer:
Didier Trono
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
10000
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
Yes
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
Kevin Ho Colin G. Nichols W. Jonathan Lederer Jonathan Lytton Peter M. Vassilev Marie V. Kanazirska & Steven C. Hebert Nature 362, 31 - 38 (1993)
Sequence: View sequences (1)
Principal Investigator:
Jay Nadeau
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: ROMK1 (Kir1.1) causes apoptosis and chronic silencing of hippocampal neurons. Nadeau et al (J Neurophysiol. 2000 Aug . 84(2):1062-75. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21322" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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