Lenti-FAB(PX-LD)
(Plasmid
#214422)
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PurposeDetection of peroxisome-lipid droplet contact site
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 214422 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneSJL CMV-GFP
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePMP34-FKBP-V5-RspAN_IRES_CFAST-20nm-Halo-6xHp NC
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SpeciesH. sapiens (human)
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Entrez GeneSLC25A17 (a.k.a. PMP34)
- Promoter CMV
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Tags
/ Fusion Proteins
- FKBP-V5-RspAN (N terminal on insert)
- CFAST-Halo (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lenti-FAB(PX-LD) was a gift from Chi-Lun Chang (Addgene plasmid # 214422 ; http://n2t.net/addgene:214422 ; RRID:Addgene_214422) -
For your References section:
A fluorogenic complementation tool kit for interrogating lipid droplet-organelle interaction. Li X, Gamuyao R, Wu ML, Cho WJ, Kurtz NB, King SV, Petersen RA, Stabley DR, Lindow C, Climer L, Shirinifard A, Ferrara F, Throm RE, Robinson CG, Carisey A, Tebo AG, Chang CL. bioRxiv. 2023 Nov 29:2023.11.29.569289. doi: 10.1101/2023.11.29.569289. Preprint. 10.1101/2023.11.29.569289 PubMed 38076863
