A fluorogenic complementation tool kit for interrogating lipid droplet-organelle interaction.
Li X, Gamuyao R, Wu ML, Cho WJ, Kurtz NB, King SV, Petersen RA, Stabley DR, Lindow C, Climer L, Shirinifard A, Ferrara F, Throm RE, Robinson CG, Carisey A, Tebo AG, Chang CL
bioRxiv. 2023 Nov 29:2023.11.29.569289. doi: 10.1101/2023.11.29.569289. Preprint. PubMed Article
Li X, Gamuyao R, Wu ML, Cho WJ, Kurtz NB, King SV, Petersen RA, Stabley DR, Lindow C, Climer L, Shirinifard A, Ferrara F, Throm RE, Robinson CG, Carisey A, Tebo AG, Chang CL
bioRxiv. 2023 Nov 29:2023.11.29.569289. doi: 10.1101/2023.11.29.569289. Preprint. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214409 | mApple-1xHp | Lipid droplet and ER localization of 1xHp of Spastin fused to mApple |
| 214410 | mApple-6xHp | Lipid droplet localization of 6xHp of Spastin fused to mApple |
| 214411 | APEX2-6xHp | Lipid droplet localization of APEX2-6xHp for electron microscopy |
| 214412 | NFAST(low)-mApple-6xHp | Lipid droplet localization of low-affinity NFAST fused to mApple-6xHp |
| 214413 | CFAST-Halotag-ER | ER localization of CFAST fused to Halotag-ER |
| 214414 | Halotag-6xHp | Lipid droplet localization of 6xHp of Spastin fused to Halotag |
| 214415 | NFAST(low)-mApple-mito | Mitochondria localization of low-affinity NFAST fused to mApple-mito |
| 214416 | CFAST-Halotag-6xHp | Lipid droplet localization of CFAST fused to Halotag-6xHp |
| 214419 | NFAST(low)-Halo-ER | ER localization of low-affinity NFAST fused to Halo-ER |
| 214420 | Lenti-FAB(ER-LD) | Detection of ER-lipid droplet contact site |
| 214421 | Lenti-FAB(mito-LD) | Detection of mito-lipid droplet contact site |
| 214422 | Lenti-FAB(PX-LD) | Detection of peroxisome-lipid droplet contact site |
| 214423 | Lenti-FAB(ER-mito) | Detection of ER-mito contact site |
| 214424 | FAB(ER-LD) | Detection of ER-lipid droplet contact site |
| 214425 | FAB(mito-LD) | Detection of mito-lipid droplet contact site |
| 214426 | FAB(PX-LD) | Detection of peroxisome-lipid droplet contact site |
| 214427 | FAB(ER-mito) | Detection of ER-mito contact site |
| 217376 | Lenti-mApple-6xHp | Lipid droplet localization of 6xHp of Spastin fused to mApple |
| 217377 | mApple-ERM | ER marker |