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GwKif2CGFP
(Plasmid #21479)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21479 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    GW
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5309
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kinesin family member C2
  • Alt name
    KifC2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2390
  • Entrez Gene
    Kifc2
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer CTTAGGCACAGCACGATGC
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please note that the insert contains KifC2, as described in the associated publication, and not Kif2C as indicated in the plasmid name.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GwKif2CGFP was a gift from Don Arnold (Addgene plasmid # 21479 ; http://n2t.net/addgene:21479 ; RRID:Addgene_21479)
  • For your References section:

    A role for Kif17 in transport of Kv4.2. Chu PJ, Rivera JF, Arnold DB. J Biol Chem. 2006 Jan 6. 281(1):365-73. 10.1074/jbc.M508897200 PubMed 16257958