GwKif2CGFP
(Plasmid #21479)

• Purpose: Expression of KifC2-GFP in mammalian cells.
• Depositing Lab: Don Arnold
• Publication: Chu et al J Biol Chem. 2006 Jan 6. 281(1):365-73.

Backbone

• Vector backbone: GW
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5309
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kinesin family member C2
• Alt name: KifC2
• Species: M. musculus (mouse)
• Insert Size (bp): 2390
• Entrez Gene: Kifc2
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: CTTAGGCACAGCACGATGC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the insert contains KifC2, as described in the associated publication, and not Kif2C as indicated in the plasmid name.