Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21487||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt namep35srj (MRG1)
SpeciesH. sapiens (human)
Insert Size (bp)1200
Entrez GeneCITED2 (a.k.a. ASD8, MRG-1, MRG1, P35SRJ, VSD2)
- Cloning method Restriction Enzyme
- 5′ cloning site ecoRI (not destroyed)
- 3′ cloning site rsaI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
Ligated into the EcoRI/EcoRV sites of pcDNA3
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SB52 was a gift from Shoumo Bhattacharya (Addgene plasmid # 21487 ; http://n2t.net/addgene:21487 ; RRID:Addgene_21487)
For your References section:Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1. Bhattacharya S, Michels CL, Leung MK, Arany ZP, Kung AL, Livingston DM. Genes Dev. 1999 Jan 1. 13(1):64-75. 10.1101/gad.13.1.64 PubMed 9887100